Journal: Scientific reports

Article Title: Disordered metabolism in mice lacking irisin.

doi: 10.1038/s41598-020-74588-7

Figure Lengend Snippet: Figure 6. Increased bone resorption in irisin lacking mice. (A, B) Sections of the distal shaft of the femur were stained with osteoprotegerin (OPG) (1:60) and receptor activator of nuclear factor-kB ligand (RANKL) (1:80) antibodies. Positive (black arrows) osteoblasts are shown, 200×. (C) Representative images of the distal metaphyseal region of the femur, together with cell counts (osteoclasts) per bone perimeter (Bpm). Red arrows, tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts, 100×, (NIS-Elements Viewer, v4.2.0; https:// www.downza.cn/soft/2751-21.html); (D) serum levels of osteocalcin TRAP (with three replicates). (Graphpad Prism, v7.0, https://www.xue51.com/soft/3932.html). Data are presented as the mean ± SEM (n = 15 per group). *P < 0.05, **P < 0.01 compared to the WT group.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) kit for osteocalcin (OCN) (E06917m), Alkaline Phosphatase (ALP) (E0200m), and Tartrate-resistant acid phosphatase (TRAP) (E08492m) were purchased from Elabscience (Wuhan, China).

Techniques: Staining

Journal: International journal of cancer

Article Title: Transcriptional profiling on chromosome 19p indicated frequent downregulation of ACP5 expression in hepatocellular carcinoma.

doi: 10.1002/ijc.20684

Figure Lengend Snippet: FIGURE 2 – Transcriptional mapping on chromosome 19p by cDNA microarray. (a) Normalized expression levels of parental tumors (PHC-2, 4, 5 and C1) examined were plotted against the cytoband locations of cDNA clones in sequential order of megabases. Relative expression levels depicted as inverted curves indicated a consistent reduced fold change by 5- to 24-fold of a cDNA clone R05288 that represented the ACP5 gene. (b) An example of hybridized image on the cDNA microarray is shown. Parental tumor was labeled with Cy3 (green color) and normal liver pool with Cy5 (red color). The bracket highlights the duplicate spots of ACP5; a strong red signal found suggested a lower level of mRNA transcripts compared to normal control.

Article Snippet: Plasmid transfection and effect on in vitro cell growth A plasmid clone encoding a full-length human ACP5 cDNA (TC119151) was acquired from OriGene (OriGene Technologies, Rockville, MD, USA).

Techniques: Microarray, Expressing, Clone Assay, Labeling, Control

Journal: International journal of cancer

Article Title: Transcriptional profiling on chromosome 19p indicated frequent downregulation of ACP5 expression in hepatocellular carcinoma.

doi: 10.1002/ijc.20684

Figure Lengend Snippet: FIGURE 3 – ACP5 expression in HCC. (a) The bar chart indicates the log2 ratio of the normalized ACP5 ex- pression in 74 primary HCC tumors relative to the level in paired adjacent nonmalignant liver tissues. (b) Repre- sentative quantitative RT-PCR prod- ucts are shown. Expression levels of ACP5 (98 bp) determined were nor- malized against the internal reference control -actin (108 bp). The ACP5 expression in the HCC tumors (H36, H40, H42 and H46) was found to be lower than that of the adjacent nonma- lignant normal liver tissues (TN36, TN40, TN42 and TN46).

Article Snippet: Plasmid transfection and effect on in vitro cell growth A plasmid clone encoding a full-length human ACP5 cDNA (TC119151) was acquired from OriGene (OriGene Technologies, Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: International journal of cancer

Article Title: Transcriptional profiling on chromosome 19p indicated frequent downregulation of ACP5 expression in hepatocellular carcinoma.

doi: 10.1002/ijc.20684

Figure Lengend Snippet: FIGURE 4 – Effect of ectopic expression of ACP5 on HCC cell growth. (a) Growth curve of HKCI-4 cells expressing empty vector (HKCI-4/Vec). A similar result was also ob- served in the experiment with mock lipo- fectamine alone (p 0.05). (b) HKCI-4 cells expressing ACP5 (HKCI-4/ACP5) displayed a significant growth reduction at day 6 compared to both control and mock experiments (p 0.001). The values are expressed as a percent- age of maximum and each point represents the average of 5 replicates derived from 2 inde- pendent experiments (mean SD).

Article Snippet: Plasmid transfection and effect on in vitro cell growth A plasmid clone encoding a full-length human ACP5 cDNA (TC119151) was acquired from OriGene (OriGene Technologies, Rockville, MD, USA).

Techniques: Expressing, Plasmid Preparation, Control, Derivative Assay

Journal: Journal of Dental Sciences

Article Title: Human umbilical cord mesenchymal stem cells secretome and nanoemulsion propolis combination ameliorate osteoclastogenesis in lipopolysaccharide-induced osteolysis in hyperglycemia rats

doi: 10.1016/j.jds.2025.07.003

Figure Lengend Snippet: Effect of NEP and HUCMSCS combination on LPS-induced osteolysis in hyperglycemic conditions in TRAPase and Ctsk serum levels. (A) Concentration of TRAP in serum was measured by ELISA. (B) Concentration of Ctsk in serum was measured by ELISA. A comparison of (A) TRAPase and (B) Ctsk serum levels in all experimental groups. Results are expressed as the mean ± SD. The statistical significance of differences was determined by t-tests and one-way analysis of variance (ANOVA) by Tukey Honest Significant Different (HSD) with significance set at P ≤ 0.05. n = 4 for each group. CTSK, Cathepsin K; HUCMSCS, human umbilical cord mesenchymal stem cells secretome; LPS, lipopolysaccharide; NEP, nanoemulsion propolis extract; PBS, phosphate-buffered saline; SD, standart deviation; TRAP, tartrate-resistant acid phosphatase.

Article Snippet: The steps of the sandwich ELISA method begin with preparation, sample incubation, washing, target antibody incubation, sample washing, analysis, and interpretation according to the instructions of the rat TRAP and Ctsk ELISA kit (Shanghai Korain Biotech Co., Ltd., Shanghai, China).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison, Saline